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Researchers develop credit-card sized tool to understand how cancer cells invade host tissues

鈥淲e think this type of tool will be quite useful to the community鈥
photo of researcher in lab
鈥淯sing the tool we developed, researchers in the future can develop therapeutics that target some of these genes to halt the cancer metastasis,鈥 Betty Li said. (photo by Michael Dryden)

A group of researchers from the University of Toronto has developed a credit-card sized tool for growing cancer cells outside the human body, which they believe will enhance their understanding of breast cancer metastasis.

The device, described in , reproduces various environments within the human body where breast cancer cells live. Studying the cells as they go through the process of invasion and metastasis could point the way toward new biomarkers and drugs to diagnose and treat cancer.

鈥淢etastasis is what makes cancer so deadly,鈥 said the publication's corresponding author  , a professor in the Institute of Biomedical Engineering in the Faculty of Applied Science & Engineering, is located in the  in 茄子直播's Faculty of Medicine.

鈥淚f cancer cells would simply stay in one spot, it would be 鈥榚asy鈥 to excise them and cure the disease. But when cancer metastasizes, cancer cells move through the body, making the disease difficult to treat.

鈥淲e decided to apply our expertise in microfluidics to develop a new tool to aid in studying how cancer cells begin to invade into surrounding tissues in the first steps in metastasis.鈥

Normally metastasis is studied in a petri dish cell culture or in whole animals. However, these model systems present problems in terms of cost, efficiency, or lack of representation.

鈥淎n oversimplified system like cells in petri dishes doesn鈥檛 mimic what happens in the body, while in an animal model, it鈥檚 difficult to isolate and study parameters that govern the invasiveness of a cell,鈥 said Betty Li, a senior PhD student and lead author of the paper.

鈥淥ur system gives us control over all the specific parameters that we want to look at, while allowing us to make structures that better resemble what happens to the body.鈥

The device consists of patterned metal electrodes which can move extremely small droplets around through the use of electric fields. By selectively changing the water-repelling properties of the surface at various points, researchers can 鈥榩inch鈥 off the water droplets and form precise shapes.

In the paper, the researchers describe how they used a collagen matrix coated with a layer of basal membrane extract to mimic the structure of the breast tissue seen by breast cancer cells during the first step of metastasis.

By placing cancer cells outside of these tissue mimics, researchers could observe the invasion process in detail, including measurements of speed and location.

鈥淥ne interesting thing we observed is that not all cancer cells within the same population have the same invasiveness,鈥 Li said. 鈥淪ome invaded into the tissue mimics while others did not, which prompted us to look at what gives the invaded cells such an advantage.鈥

Li and her team extracted cancer cells at various distances from the invasion point and subjected these cells to genetic sequencing.

鈥淲e identified 244 different genes that are differentially expressed between the cancer cells that invaded versus the ones that didn鈥檛 invade,鈥 Li said. 鈥淭his means that using the tool we developed, researchers in the future can develop therapeutics that target some of these genes to halt the cancer metastasis.鈥

鈥淲e think this type of tool will be quite useful to the community, as cell invasion is important in cancer and also a host of other (non-pathological) processes, like tissue growth, differentiation and repair,鈥 Wheeler said.

This research was funded by the National Sciences and Engineering Research Council of Canada, the Canada Foundation for Innovation, the Province of Ontario, and by 茄子直播's Medicine by Design initiative, which receives funding from the Canada First Research Excellence Fund.

Engineering